Multiphoton Fluorescence Microscopy
ثبت نشده
چکیده
The human body is a constant flux of thousands of chemical/biological interactions and processes connecting molecules, cells, organs, and fluids, throughout the brain, body, and nervous system. Up until recently it was thought that all these interactions operated in a linear sequence, passing on information much like a runner passing the baton to the next runner. However, the latest findings in quantum biology and biophysics have discovered that there is in fact a tremendous degree of coherence within all living systems.
منابع مشابه
Multifocal multiphoton microscopy: a fast and efficient tool for 3-D fluorescence imaging
Multifocal multiphoton microscopy (MMM) is an efficient and technically simple method for generating multiphoton fluorescence images. Featuring the high axial resolution of confocal and multiphoton scanning microscopes, MMM also achieves high speed in 3-D microscopy. In this paper, examples of fast-mode 3-D microscopy are given including imaging of fixed brain tissue as well as living PC12 cell...
متن کاملOptimizing 3D multiphoton fluorescence microscopy.
We present a new optimization concept for 3D multiphoton fluorescence microscopy by finding the optimal excitation beam giving rise to the smallest possible light-emitting volume or the highest possible signal to noise ratio (SNR).
متن کاملSpectral-domain optical coherence phase and multiphoton microscopy.
We describe simultaneous quantitative phase contrast and multiphoton fluorescence imaging by combined spectral-domain optical coherence phase and multiphoton microscopy. The instrument employs two light sources for efficient optical coherence microscopic and multiphoton imaging and can generate structural and functional images of transparent specimens in the epidirection. Phase contrast imaging...
متن کاملFunctional studies in living animals using multiphoton microscopy.
In vivo microscopy is a powerful method for studying fundamental issues of physiology and pathophysiology. The recent development of multiphoton fluorescence microscopy has extended the reach of in vivo microscopy, supporting high-resolution imaging deep into the tissues and organs of living animals. As compared with other in vivo imaging techniques, multiphoton microscopy is uniquely capable o...
متن کاملMultiphoton endoscopy.
Despite widespread use of multiphoton fluorescence microscopy, development of endoscopes for nonlinear optical imaging has been stymied by the degradation of ultrashort excitation pulses that occurs within optical fiber as a result of the combined effects of group-velocity dispersion and self-phase modulation. We introduce microendoscopes (350-1000 microm in diameter) based on gradient-index mi...
متن کاملQuantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera.
Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak camera is described. An example of a typical application of the system is pro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2016